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A highthroughput production of composite breast tumoroids: a tool for investigation of cellular heterogeneity and drug delivery
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|Title: ||A highthroughput production of composite breast tumoroids: a tool for investigation of cellular heterogeneity and drug delivery|
|Authors: ||Vamvakidou, Alexandra P.|
Mondrinos, Mark J.
Petushi, Sokol P.
Lelkes, Peter I.
|Issue Date: ||2006 |
|Citation: ||Poster presented at Biomedical Technology Showcase 2006, Philadelphia, PA. Retrieved 18 Aug 2006 from http://www.biomed.drexel.edu/new04/Content/Biomed_Tech_Showcase/Poster_Presentations/Tozeren.pdf.|
|Abstract: ||Breast tumors are typically heterogeneous and contain diverse subpopulations of tumor cells with differing phenotypic properties. This study has developed an in vitro co-culture-based three-dimensional breast tumor model that studies the effects of mixing heterogeneous tumor cell populations. Breast cancer cell lines of different phenotypes (MDAMB231, MCF7 and ZR751) were co-cultured in a rotating wall vessel (RWV) bioreactor to form a large number of heterogeneous tumoroids. Prior to each experiment, cells were labeled with cell tracker dyes to allow for time-course fluorescence microscopy to monitor cell aggregation. Histological sections of the tumor spheroids were stained with hematoxylin and eosin (HE), progesterone receptor (PR), E-cadherin (E-cad) and proliferation marker, ki67. Results showed that heterogeneous tumoroids reflecting the composition of the growth rate, invasion potential, and spatial distributions of heterogeneous tumor spheroids were highly dependent on cell composition. A suitable in vitro model for studying tumor-cell heterogeneity and reciprocal interactions will accelerate understanding of tumor cell phenotype population dynamics.|
|Description: ||Item from the Biomedical Technology Showcase held July 20, 2006 at Drexel University's Bossone Research Enterprise Center.|
|Appears in Collections:||Biomedical Technology Showcase, 2006|
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