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Imaging biomarkers of inflammation in situ with functionalized quantum dots in the dextran sodium sulfate (DSS) model of mouse colitis
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|Title: ||Imaging biomarkers of inflammation in situ with functionalized quantum dots in the dextran sodium sulfate (DSS) model of mouse colitis|
|Authors: ||Karwa, Amol|
Papazoglou, Elisabeth S.
|Keywords: ||Inflammation;Colitis;Animal Model of Colitis;Quantum Dots;Myeloperoxidase;extran Sodium Sulfate;TNFα;IL1α;Nanotechnology|
|Issue Date: ||2007|
|Publisher: ||Springer Verlag|
|Citation: ||Inflammation Research, 56(12): pp. 502-510.|
|Abstract: ||Objective and Design: Myeloperoxidase (MPO) and proinflammatory cytokines play an important role in the development of inflammation. These markers are generally measured using tedious ELISA procedures. In this study, a novel technique utilizing antibody conjugated quantum dot nanoparticles was developed to detect myeloperoxidase, IL-1 α and TNF-α in vivo in the dextran sodium sulfate (DSS) model of experimental colitis. Materials and Methods: Colitis was induced in animals (n=8 animals/ group) by feeding 4% DSS solution ad libitum for seven to eight days. Quantum Dots exhibiting fluorescence at various wavelengths were conjugated to MPO, IL-1 α and TNF-α polyclonal antibodies and tested in vivo at various stages of colitis. Tissue sections obtained were imaged with confocal microscope. The image intensity obtained from the tissue specimen was correlated with clinical activity measured as Disease Activity Index (DAI).
Results: Myeloperoxidase, IL-1α and TNF-α were visualized with quantum dots on various days of disease. The intensity of quantum dots increased with increase in inflammation. The increase in intensity showed an excellent correlation with the DAI based on the clinical parameters.
Conclusion: The study demonstrated that multiple biomarkers can be detected simultaneously and their quantitative expression correlated well with clinical disease severity. This novel technology should facilitate design of a novel optical platform for imaging various biomarkers of inflammation, early detection of acute and chronic disease markers and inflammation-mediated cancer markers. This detection may also facilitate determination of therapeutic success.|
|Appears in Collections:||Faculty Research and Publications (Physics)|
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